› 4i9b

X-ray diffraction
1.9Å resolution

Structure of aminoaldehyde dehydrogenase 1 from Solanum lycopersium (SlAMADH1) with a thiohemiacetal intermediate

Released:
DOI: 10.2210/pdb4i9b/pdb
PDB entry 4i9b coloured by chain, front view.
PDB entry 4i9b coloured by chain, side view.
PDB entry 4i9b coloured by chain, top view.
Source organism: Solanum lycopersicum
Primary publication:
Plant ALDH10 family: identifying critical residues for substrate specificity and trapping a thiohemiacetal intermediate.
Kopečny D, Končitíková R, Tylichová M, Vigouroux A, Moskalíková H, Soural M, Šebela M, Moréra S
J Biol Chem 288 9491-507 (2013)
PMID: 23408433

Function and Biology Details

Reactions catalysed: 
4-aminobutanal + NAD(+) + H(2)O = 4-aminobutanoate + NADH
4-trimethylammoniobutanal + NAD(+) + H(2)O = 4-trimethylammoniobutanoate + NADH
4-guanidinobutanal + NAD(+) + H(2)O = 4-guanidinobutanoate + NADH
Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176392 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoaldehyde dehydrogenase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 517 amino acids
Theoretical weight: 56.35 KDa
Source organism: Solanum lycopersicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56R04 (Residues: 2-504; Coverage: 100%)
Gene names: ALDH10A12, AMADH1, Solyc06g071290
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
4 bound ligands:
Ligand NA 2 x NA
Ligand PEG 2 x PEG
Ligand EDO 1 x EDO
Ligand 1KA 2 x 1KA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 84.15Å b: 94.79Å c: 118.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.174 0.212
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Recent Development on Plant Aldehyde Dehydrogenase Enzymes and Their Functions in Plant Development and Stress Signaling.
Tola et al. (2020)
1 more

6 mentions without citation

Plant ALDH10 family: identifying critical residues for substrate specificity and trapping a thiohemiacetal intermediate.
Kopečny et al. (2013)
5 more