› 4i9s

X-ray diffraction
2.58Å resolution

Crystal Structure of the R111K:R132L:Y134F:T54V:R59W Mutant of the Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans Retinal at 2.58 Angstrom Resolution

Released:
DOI: 10.2210/pdb4i9s/pdb
PDB entry 4i9s coloured by chain, front view.
PDB entry 4i9s coloured by chain, side view.
PDB entry 4i9s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Rational design of a colorimetric pH sensor from a soluble retinoic acid chaperone.
Berbasova T, Nosrati M, Vasileiou C, Wang W, Lee KS, Yapici I, Geiger JH, Borhan B
J Am Chem Soc 135 16111-9 (2013)
PMID: 24059243

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular retinoic acid-binding protein 2 Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29373 (Residues: 2-138; Coverage: 99%)
Gene name: CRABP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P3121
Unit cell:
a: 56.455Å b: 56.455Å c: 108.192Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.224 0.288
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Cyanine polyene reactivity: scope and biomedical applications.
Gorka et al. (2015)
1 more

5 mentions without citation

AlphaFill: enriching AlphaFold models with ligands and cofactors.
Hekkelman et al. (2023)
4 more