› 4ia4

X-ray diffraction
3.1Å resolution

Structure of the spinach aquaporin SoPIP2;1 at pH 6

Released:
DOI: 10.2210/pdb4ia4/pdb
PDB entry 4ia4 coloured by chain, front view.
PDB entry 4ia4 coloured by chain, side view.
PDB entry 4ia4 coloured by chain, top view.
Source organism: Spinacia oleracea
Primary publication:
Structural basis for pH gating of plant aquaporins.
Frick A, Järvå M, Törnroth-Horsefield S
FEBS Lett 587 989-93 (2013)
PMID: 23454640

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aquaporin PIP-type Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 281 amino acids
Theoretical weight: 29.93 KDa
Source organism: Spinacia oleracea
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q41372 (Residues: 1-281; Coverage: 100%)
Gene name: LOC110800173
Sequence domains: Major intrinsic protein
Structure domains: Glycerol uptake facilitator protein.

Ligands and Environments

1 bound ligand:
Ligand HG 12 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 178.227Å b: 104.19Å c: 66.523Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.243
Expression system: Komagataella pastoris

Quick links

Citations

12 review citations

Aquaporins in Plants.
Maurel et al. (2015)
11 more

2 mentions without citation

Molecular mechanisms governing aquaporin relocalisation.
Markou et al. (2022)
1 more