› 4ia6

X-ray diffraction
1.8Å resolution

Hydratase from lactobacillus acidophilus in a ligand bound form (LA LAH)

Released:
DOI: 10.2210/pdb4ia6/pdb
PDB entry 4ia6 coloured by chain, front view.
PDB entry 4ia6 coloured by chain, side view.
PDB entry 4ia6 coloured by chain, top view.
Source organism: Lactobacillus acidophilus NCFM
Primary publication:
Crystal structure analysis of a fatty acid double-bond hydratase from Lactobacillus acidophilus.
Volkov A, Khoshnevis S, Neumann P, Herrfurth C, Wohlwend D, Ficner R, Feussner I
Acta Crystallogr D Biol Crystallogr 69 648-57 (2013)
PMID: 23519674

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177110 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin-crossreactive antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 591 amino acids
Theoretical weight: 67.7 KDa
Source organism: Lactobacillus acidophilus NCFM
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5FL96 (Residues: 1-591; Coverage: 100%)
Gene name: LBA0649
Sequence domains: MCRA family
Structure domains:

Ligands and Environments

10 bound ligands:
Ligand MRD 1 x MRD
Ligand MPD 5 x MPD
Ligand GOL 10 x GOL
Ligand EIC 1 x EIC
Ligand NA 2 x NA
Ligand PO4 4 x PO4
Ligand MES 3 x MES
Ligand K 2 x K
Ligand TRS 1 x TRS
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21212
Unit cell:
a: 165.1Å b: 77.74Å c: 104.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.192
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Microbial Production of Conjugated Linoleic Acid and Conjugated Linolenic Acid Relies on a Multienzymatic System.
Salsinha et al. (2018)
4 more

4 mentions without citation

Novel oleate hydratases and potential biotechnological applications.
Hagedoorn et al. (2021)
3 more