› 4iah

X-ray diffraction
2.8Å resolution

Crystal Structure of BAY 60-2770 bound C139A H-NOX domain with S-nitrosylated conserved C122

Released:
DOI: 10.2210/pdb4iah/pdb
PDB entry 4iah coloured by chain, front view.
PDB entry 4iah coloured by chain, side view.
PDB entry 4iah coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Primary publication:
Insights into BAY 60-2770 activation and S-nitrosylation-dependent desensitization of soluble guanylyl cyclase via crystal structures of homologous nostoc H-NOX domain complexes.
Kumar V, Martin F, Hahn MG, Schaefer M, Stamler JS, Stasch JP, van den Akker F
Biochemistry 52 3601-8 (2013)
PMID: 23614626

Function and Biology Details

Reaction catalysed: 
GTP = 3',5'-cyclic GMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-187121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme NO-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.39 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8YUQ7 (Residues: 1-182; Coverage: 96%)
Gene name: alr2278
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

2 bound ligands:
Ligand 1DX 2 x 1DX
Ligand MLI 2 x MLI
1 modified residue:
Ligand SNC 2 x SNC

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P213
Unit cell:
a: 122.573Å b: 122.573Å c: 122.573Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.163 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Thirty Years of Saying NO: Sources, Fate, Actions, and Misfortunes of the Endothelium-Derived Vasodilator Mediator.
Vanhoutte et al. (2016)
15 more

2 mentions without citation

Insights into BAY 60-2770 activation and S-nitrosylation-dependent desensitization of soluble guanylyl cyclase via crystal structures of homologous nostoc H-NOX domain complexes.
Kumar et al. (2013)
1 more