› 4if4

X-ray diffraction
2.35Å resolution

Crystal Structure of the Magnesium and beryllofluoride-activated VraR from Staphylococcus aureus

Released:
DOI: 10.2210/pdb4if4/pdb
PDB entry 4if4 coloured by chain, front view.
PDB entry 4if4 coloured by chain, side view.
PDB entry 4if4 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
Phosphorylation-dependent conformational changes and domain rearrangements in Staphylococcus aureus VraR activation.
Leonard PG, Golemi-Kotra D, Stock AM
Proc Natl Acad Sci U S A 110 8525-30 (2013)
PMID: 23650349

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulator protein VraR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 208 amino acids
Theoretical weight: 23.46 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7A2Q1 (Residues: 2-209; Coverage: 100%)
Gene names: SAV1884, vraR
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand BEF 4 x BEF
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R3
Unit cell:
a: 110.603Å b: 110.603Å c: 284.292Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
3 more

10 mentions without citation

Towards the computational design of protein post-translational regulation.
Strumillo et al. (2015)
9 more