› 4ig7

X-ray diffraction
2Å resolution

Crystal structure of Trichinella spiralis UCH37 bound to Ubiquitin vinyl methyl ester

Released:
DOI: 10.2210/pdb4ig7/pdb
PDB entry 4ig7 coloured by chain, front view.
PDB entry 4ig7 coloured by chain, side view.
PDB entry 4ig7 coloured by chain, top view.
Source organisms:
Primary publication:
Stabilization of an unusual salt bridge in ubiquitin by the extra C-terminal domain of the proteasome-associated deubiquitinase UCH37 as a mechanism of its exo specificity.
Morrow ME, Kim MI, Ronau JA, Sheedlo MJ, White RR, Chaney J, Paul LN, Lill MA, Artavanis-Tsakonas K, Das C
Biochemistry 52 3564-78 (2013)
PMID: 23617878

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143374 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin C-terminal hydrolase 37 Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 38.81 KDa
Source organism: Trichinella spiralis
Expression system: Escherichia coli
Structure domains: Peptidase C12, ubiquitin carboxyl-terminal hydrolase
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 75 amino acids
Theoretical weight: 8.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-683; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand GVE 1 x GVE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: R3
Unit cell:
a: 147.361Å b: 147.361Å c: 40.485Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.24
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Molecular architecture of polycomb repressive complexes.
Chittock et al. (2017)
12 other articles

6 mentions without citation

Substrate specificity of the ubiquitin and Ubl proteases.
Ronau et al. (2016)
5 more