› 4ig8

X-ray diffraction
2.7Å resolution

Structural basis for cytosolic double-stranded RNA surveillance by human OAS1

Released:
DOI: 10.2210/pdb4ig8/pdb
PDB entry 4ig8 coloured by chain, front view.
PDB entry 4ig8 coloured by chain, side view.
PDB entry 4ig8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for cytosolic double-stranded RNA surveillance by human oligoadenylate synthetase 1.
Donovan J, Dufner M, Korennykh A
Proc Natl Acad Sci U S A 110 1652-7 (2013)
PMID: 23319625

Function and Biology Details

Reaction catalysed: 
3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133748 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
2'-5'-oligoadenylate synthase 1 Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 40.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00973 (Residues: 1-347; Coverage: 87%)
Gene names: OAS1, OIAS
Sequence domains:
Structure domains:
RNA (5'-R(*GP*GP*CP*UP*UP*UP*UP*GP*AP*CP*CP*UP*UP*UP*AP*UP*GP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 18 nucleotides
Theoretical weight: 5.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
RNA (5'-R(*GP*CP*AP*UP*AP*AP*AP*GP*GP*UP*CP*AP*AP*AP*AP*GP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.8 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand DTP 1 x DTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 71.2Å b: 93.2Å c: 98.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.283
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

22 review citations

Dynamic light scattering: a practical guide and applications in biomedical sciences.
Stetefeld et al. (2016)
21 more

36 mentions without citation

Double-Stranded RNA Sensors and Modulators in Innate Immunity.
Hur S. (2019)
35 more