› 4ih4

X-ray diffraction
3.5Å resolution

Crystal structure of Arabidopsis DWARF14 orthologue, AtD14

Released:
DOI: 10.2210/pdb4ih4/pdb
PDB entry 4ih4 coloured by chain, front view.
PDB entry 4ih4 coloured by chain, side view.
PDB entry 4ih4 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structures of two phytohormone signal-transducing α/β hydrolases: karrikin-signaling KAI2 and strigolactone-signaling DWARF14.
Zhao LH, Zhou XE, Wu ZS, Yi W, Xu Y, Li S, Xu TH, Liu Y, Chen RZ, Kovach A, Kang Y, Hou L, He Y, Xie C, Song W, Zhong D, Xu Y, Wang Y, Li J, Zhang C, Melcher K, Xu HE
Cell Res 23 436-9 (2013)
PMID: 23381136

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strigolactone esterase D14 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 29.65 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SQR3 (Residues: 1-267; Coverage: 100%)
Gene names: At3g03990, D14, T11I18.10
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 184.856Å b: 44.629Å c: 160.967Å
α: 90° β: 121.07° γ: 90°
R-values:
R R work R free
0.278 0.278 0.336
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

45 review citations

Strigolactones, a novel carotenoid-derived plant hormone.
Al-Babili et al. (2015)
44 more

10 mentions without citation

Target sites for chemical regulation of strigolactone signaling.
Nakamura et al. (2014)
9 more