› 4ihc

X-ray diffraction
2Å resolution

Crystal structure of probable mannonate dehydratase Dd703_0947 (target EFI-502222) from Dickeya dadantii Ech703

Released:
DOI: 10.2210/pdb4ihc/pdb
PDB entry 4ihc coloured by chain, front view.
PDB entry 4ihc coloured by chain, side view.
PDB entry 4ihc coloured by chain, top view.
Source organism: Musicola paradisiaca Ech703
Entry authors: Patskovsky Y, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Al Obaidi NF, Stead M, Love J, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reactions catalysed: 
D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactonate dehydratase family member Dd703_0947 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 440 amino acids
Theoretical weight: 48.89 KDa
Source organism: Musicola paradisiaca Ech703
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C6CBG9 (Residues: 1-417; Coverage: 100%)
Gene name: Dd703_0947
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 8 x MG
Ligand GOL 14 x GOL
Ligand FMT 9 x FMT
Ligand IOD 19 x IOD
Ligand CL 9 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 224.331Å b: 224.347Å c: 87.076Å
α: 90° β: 97.96° γ: 90°
R-values:
R R work R free
0.175 0.173 0.228
Expression system: Escherichia coli BL21(DE3)

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