› 4ihx

X-ray diffraction
2.8Å resolution

Crystal structure of Fis bound to 27 bp 2-Aminopurine substituted DNA F28-2AP (AAATTTGTTTGA2T2TTGAGCAAATTT)

Released:
DOI: 10.2210/pdb4ihx/pdb
PDB entry 4ihx coloured by chain, front view.
PDB entry 4ihx coloured by chain, side view.
PDB entry 4ihx coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Control of DNA minor groove width and Fis protein binding by the purine 2-amino group.
Hancock SP, Ghane T, Cascio D, Rohs R, Di Felice R, Johnson RC
Nucleic Acids Res 41 6750-60 (2013)
PMID: 23661683

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-117646 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding protein fis Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
Structure domains: Homeodomain-like
27-bp DNA Strand A Chain: C
Molecule details ›
Chain: C
Length: 27 nucleotides
Theoretical weight: 8.34 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
27-bp DNA Strand B Chain: D
Molecule details ›
Chain: D
Length: 27 nucleotides
Theoretical weight: 8.24 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 2PR 3 x 2PR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 43.12Å b: 91.59Å c: 154.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.216 0.259
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Absence of a simple code: how transcription factors read the genome.
Slattery et al. (2014)
4 more

1 mention without citation

Control of DNA minor groove width and Fis protein binding by the purine 2-amino group.
Hancock et al. (2013)