› 4iil

X-ray diffraction
1.3Å resolution

Crystal Structure of RfuA (TP0298) of T. pallidum Bound to Riboflavin

Released:
DOI: 10.2210/pdb4iil/pdb
PDB entry 4iil coloured by chain, front view.
PDB entry 4iil coloured by chain, side view.
PDB entry 4iil coloured by chain, top view.
Source organism: Treponema pallidum subsp. pallidum str. Nichols
Primary publication:
Evidence for an ABC-type riboflavin transporter system in pathogenic spirochetes.
Deka RK, Brautigam CA, Biddy BA, Liu WZ, Norgard MV
mBio 4 e00615-12 (2013)
PMID: 23404400

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC transporter riboflavin-binding protein RfuA Chain: A
Molecule details ›
Chain: A
Length: 346 amino acids
Theoretical weight: 38.48 KDa
Source organism: Treponema pallidum subsp. pallidum str. Nichols
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q56328 (Residues: 21-343; Coverage: 100%)
Gene names: TP_0298, rfuA, tpn38
Sequence domains: ABC transporter substrate-binding protein PnrA-like
Structure domains: Rossmann fold

Ligands and Environments

4 bound ligands:
Ligand RBF 1 x RBF
Ligand NA 2 x NA
Ligand K 1 x K
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 75.955Å b: 107.79Å c: 72.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.121 0.153
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Overlapping riboflavin supply pathways in bacteria.
García-Angulo VA. (2017)
3 more

3 mentions without citation

Evidence for an ABC-type riboflavin transporter system in pathogenic spirochetes.
Deka et al. (2013)
2 more