› 4ijj

X-ray diffraction
3.25Å resolution

Structure of transcription factor DksA2 from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4ijj/pdb
PDB entry 4ijj coloured by chain, front view.
PDB entry 4ijj coloured by chain, side view.
PDB entry 4ijj coloured by chain, top view.
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Primary publication:
DksA2, a zinc-independent structural analog of the transcription factor DksA.
Furman R, Biswas T, Danhart EM, Foster MP, Tsodikov OV, Artsimovitch I
FEBS Lett 587 614-9 (2013)
PMID: 23416301

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase-binding transcription factor DksA Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 136 amino acids
Theoretical weight: 16.23 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2ZJP8 (Residues: 1-134; Coverage: 100%)
Gene names: PA14_73020, dksA
Sequence domains:
Structure domains: DksA, coiled-coil domain

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P622
Unit cell:
a: 169.453Å b: 169.453Å c: 92.32Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.259 0.257 0.31
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Transcriptional Responses to ppGpp and DksA.
Gourse et al. (2018)
1 more

6 mentions without citation

DksA2, a zinc-independent structural analog of the transcription factor DksA.
Furman et al. (2013)
5 more