› 4ijs

X-ray diffraction
3.2Å resolution

Crystal structure of nucleocapsid protein encoded by the prototypic member of orthobunyavirus

Released:
DOI: 10.2210/pdb4ijs/pdb
PDB entry 4ijs coloured by chain, front view.
PDB entry 4ijs coloured by chain, side view.
PDB entry 4ijs coloured by chain, top view.
Source organism: Bunyamwera virus
Primary publication:
Bunyamwera virus possesses a distinct nucleocapsid protein to facilitate genome encapsidation.
Li B, Wang Q, Pan X, Fernández de Castro I, Sun Y, Guo Y, Tao X, Risco C, Sui SF, Lou Z
Proc Natl Acad Sci U S A 110 9048-53 (2013)
PMID: 23569257

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147748 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleoprotein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 248 amino acids
Theoretical weight: 28.4 KDa
Source organism: Bunyamwera virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P16495 (Residues: 2-233; Coverage: 100%)
Gene name: N
Sequence domains: Bunyavirus nucleocapsid (N) protein
Structure domains:
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 10 nucleotides
Theoretical weight: 3.25 KDa
Source organism: Bunyamwera virus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: I422
Unit cell:
a: 106.14Å b: 106.14Å c: 485.187Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

The SARS coronavirus nucleocapsid protein--forms and functions.
Chang et al. (2014)
9 more

7 mentions without citation

Nucleocapsid Structure of Negative Strand RNA Virus.
Luo et al. (2020)
6 more