› 4ik5

X-ray diffraction
2.5Å resolution

High resolution structure of Delta-REST-GCaMP3

Released:
DOI: 10.2210/pdb4ik5/pdb
PDB entry 4ik5 coloured by chain, front view.
PDB entry 4ik5 coloured by chain, side view.
PDB entry 4ik5 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into enhanced calcium indicator GCaMP3 and GCaMPJ to promote further improvement.
Chen Y, Song X, Ye S, Miao L, Zhu Y, Zhang RG, Ji G
Protein Cell 4 299-309 (2013)
PMID: 23549615

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EF-hand domain-containing protein; Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 46.76 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: K4DIE3 (Residues: 25-48, 51-59, 60-94, 95-127, 128-147, 279-283, 306-338, 342-354, 361-362, 368-432; Coverage: 52%)
  • Canonical: P42212 (Residues: 2-141, 145-170, 185-187, 201-206, 209-213; Coverage: 74%)
Gene name: GFP
Sequence domains:
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P41212
Unit cell:
a: 120.021Å b: 120.021Å c: 96.876Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.176 0.228
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Beyond neurovascular coupling, role of astrocytes in the regulation of vascular tone.
Filosa et al. (2016)
6 more

2 mentions without citation

Deciphering the molecular mechanism responsible for GCaMP6m's Ca2+-dependent change in fluorescence.
Barnett et al. (2017)
1 more