› 4ikh

X-ray diffraction
2.1Å resolution

Crystal structure of a glutathione transferase family member from Pseudomonas fluorescens pf-5, target efi-900003, with two glutathione bound

Released:
DOI: 10.2210/pdb4ikh/pdb
PDB entry 4ikh coloured by chain, front view.
PDB entry 4ikh coloured by chain, side view.
PDB entry 4ikh coloured by chain, top view.
Source organism: Pseudomonas protegens Pf-5
Entry authors: Vetting MW, Sauder JM, Morisco LL, Wasserman SR, Sojitra S, Imker HJ, Burley SK, Armstrong RN, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 27.92 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4KED9 (Residues: 2-234; Coverage: 100%)
Gene name: PFL_2287
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P6122
Unit cell:
a: 88.189Å b: 88.189Å c: 159.834Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.207 0.234
Expression system: Escherichia coli BL21(DE3)

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