› 4ikm

X-ray diffraction
2.46Å resolution

X-ray structure of CARD8 CARD domain

Released:
DOI: 10.2210/pdb4ikm/pdb
PDB entry 4ikm coloured by chain, front view.
PDB entry 4ikm coloured by chain, side view.
PDB entry 4ikm coloured by chain, top view.
Source organisms:
Primary publication:
The structure of the CARD8 caspase-recruitment domain suggests its association with the FIIND domain and procaspases through adjacent surfaces.
Jin T, Huang M, Smith P, Jiang J, Xiao TS
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 482-7 (2013)
PMID: 23695559

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142608 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Caspase recruitment domain-containing protein 8, C-terminus; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 469 amino acids
Theoretical weight: 53.37 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: Q9Y2G2 (Residues: 451-537; Coverage: 16%)
Gene names: CARD8, DACAR, JW3994, KIAA0955, NDPP1, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand IOD 2 x IOD
Ligand EDO 2 x EDO
2 modified residues:
Ligand IYR 3 x IYR
Ligand TYI 5 x TYI

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P6522
Unit cell:
a: 94.877Å b: 94.877Å c: 219.39Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.253
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

The versatile roles of CARDs in regulating apoptosis, inflammation, and NF-κB signaling.
Kao et al. (2015)
6 other articles

7 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
6 more