› 4iky

X-ray diffraction
2.1Å resolution

Crystal structure of peptide transporter POT (E310Q mutant) in complex with sulfate

Released:
DOI: 10.2210/pdb4iky/pdb
PDB entry 4iky coloured by chain, front view.
PDB entry 4iky coloured by chain, side view.
PDB entry 4iky coloured by chain, top view.
Source organism: Geobacillus kaustophilus
Primary publication:
Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT.
Doki S, Kato HE, Solcan N, Iwaki M, Koyama M, Hattori M, Iwase N, Tsukazaki T, Sugita Y, Kandori H, Newstead S, Ishitani R, Nureki O
Proc Natl Acad Sci U S A 110 11343-8 (2013)
PMID: 23798427

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177388 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major facilitator superfamily (MFS) profile domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 507 amino acids
Theoretical weight: 54.77 KDa
Source organism: Geobacillus kaustophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KYD1 (Residues: 1-496; Coverage: 100%)
Gene name: GK2020
Sequence domains: POT family
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

3 bound ligands:
Ligand SO4 7 x SO4
Ligand OLA 6 x OLA
Ligand OLC 2 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P21
Unit cell:
a: 52.637Å b: 93.536Å c: 57.507Å
α: 90° β: 112.38° γ: 90°
R-values:
R R work R free
0.216 0.214 0.248
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
19 more

6 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
5 more