› 4ilh

X-ray diffraction
1.85Å resolution

Crystal structure of an Aar2p C-terminal deletion mutant in conplex with Prp8p RNaseH

Released:
DOI: 10.2210/pdb4ilh/pdb
PDB entry 4ilh coloured by chain, front view.
PDB entry 4ilh coloured by chain, side view.
PDB entry 4ilh coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Entry authors: Weber G, Heroven AC, Santos KF, Wahl MC

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152275 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pre-mRNA-splicing factor 8 Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 29.5 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P33334 (Residues: 1836-2090; Coverage: 11%)
Gene names: DBF3, DNA39, PRP8, RNA8, SLT21, USA2, YHR165C
Sequence domains: PRP8 domain IV core
Structure domains:
A1 cistron-splicing factor AAR2 Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 39.96 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32357 (Residues: 1-331; Coverage: 93%)
Gene names: AAR2, YBL06.06, YBL0611, YBL074C
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 79.89Å b: 76.76Å c: 91.86Å
α: 90° β: 105.88° γ: 90°
R-values:
R R work R free
0.17 0.168 0.217
Expression system: Escherichia coli

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