› 4ilk

X-ray diffraction
2Å resolution

Crystal structure of short chain alcohol dehydrogenase (rspB) from E. coli CFT073 (EFI TARGET EFI-506413) complexed with cofactor NADH

Released:
DOI: 10.2210/pdb4ilk/pdb
PDB entry 4ilk coloured by chain, front view.
PDB entry 4ilk coloured by chain, side view.
PDB entry 4ilk coloured by chain, top view.
Source organism: Escherichia coli CFT073
Entry authors: Lukk T, Wichelecki D, Imker HJ, Gerlt JA, Nair SK

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101546 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Starvation sensing protein rspB Chains: A, B
Molecule details ›
Chains: A, B
Length: 359 amino acids
Theoretical weight: 38.72 KDa
Source organism: Escherichia coli CFT073
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2V9Q5 (Residues: 9-347; Coverage: 98%)
Gene names: c1970, rspB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NAI 2 x NAI
Ligand MN 2 x MN
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 47.322Å b: 97.133Å c: 144.101Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.165 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links