› 4ime

X-ray diffraction
1.75Å resolution

Crystal Structure of Pasteurella multocida N-Acetyl-D-Neuraminic acid lyase K164A Mutant

Released:
DOI: 10.2210/pdb4ime/pdb
PDB entry 4ime coloured by chain, front view.
PDB entry 4ime coloured by chain, side view.
PDB entry 4ime coloured by chain, top view.
Source organism: Pasteurella multocida subsp. gallicida P1059
Primary publication:
Structural basis for substrate specificity and mechanism of N-acetyl-D-neuraminic acid lyase from Pasteurella multocida.
Huynh N, Aye A, Li Y, Yu H, Cao H, Tiwari VK, Shin DW, Chen X, Fisher AJ
Biochemistry 52 8570-9 (2013)
PMID: 24152047

Function and Biology Details

Reaction catalysed: 
Aceneneuramate = N-acetyl-D-mannosamine + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189981 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylneuraminate lyase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 293 amino acids
Theoretical weight: 32.58 KDa
Source organism: Pasteurella multocida subsp. gallicida P1059
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CKB0 (Residues: 1-293; Coverage: 100%)
Gene names: PM1715, nanA
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand ME2 7 x ME2
Ligand PG6 1 x PG6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 90.14Å b: 147.71Å c: 111.98Å
α: 90° β: 98.51° γ: 90°
R-values:
R R work R free
0.177 0.176 0.208
Expression system: Escherichia coli BL21(DE3)

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Citations

6 citation in other articles

Elucidation of a sialic acid metabolism pathway in mucus-foraging Ruminococcus gnavus unravels mechanisms of bacterial adaptation to the gut.
Bell et al. (2019)
5 more

3 mentions without citation

Biochemical and structural basis of sialic acid utilization by gut microbes.
Bell et al. (2023)
2 more