› 4imv

X-ray diffraction
2.25Å resolution

Ricin A-chain variant 1-33/44-198 with engineered disulfide bond, R48C/T77C/D75N

Released:
DOI: 10.2210/pdb4imv/pdb
PDB entry 4imv coloured by chain, front view.
PDB entry 4imv coloured by chain, side view.
PDB entry 4imv coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
Disruption of the putative vascular leak peptide sequence in the stabilized ricin vaccine candidate RTA1-33/44-198.
Janosi L, Compton JR, Legler PM, Steele KE, Davis JM, Matyas GR, Millard CB
Toxins (Basel) 5 224-48 (2013)
PMID: 23364220

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin A chain Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.41 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02879 (Residues: 36-233; Coverage: 35%)
Sequence domains: Ribosome inactivating protein
Structure domains: Ricin (A subunit), domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR-H
Spacegroup: I222
Unit cell:
a: 51.585Å b: 72.336Å c: 94.694Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Progress and challenges associated with the development of ricin toxin subunit vaccines.
Vance et al. (2016)
1 more

2 mentions without citation

Stability of isolated antibody-antigen complexes as a predictive tool for selecting toxin neutralizing antibodies.
Legler et al. (2017)
1 more