› 4ina

X-ray diffraction
2.49Å resolution

Crystal Structure of the Q7MSS8_WOLSU protein from Wolinella succinogenes. Northeast Structural Genomics Consortium Target WsR35

Released:
DOI: 10.2210/pdb4ina/pdb
PDB entry 4ina coloured by chain, front view.
PDB entry 4ina coloured by chain, side view.
PDB entry 4ina coloured by chain, top view.
Source organism: Wolinella succinogenes
Entry authors: Vorobiev S, Su M, Seetharaman J, Maglaqui M, Xiao R, Kohan E, Wang D, Everett JK, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD(+) + H(2)O = L-lysine + 2-oxoglutarate + NADH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Saccharopine dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 405 amino acids
Theoretical weight: 46.66 KDa
Source organism: Wolinella succinogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7MSS8 (Residues: 1-397; Coverage: 100%)
Gene name: WS0167
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 44.639Å b: 95.49Å c: 104.427Å
α: 90° β: 101.8° γ: 90°
R-values:
R R work R free
0.195 0.193 0.222
Expression system: Escherichia coli

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