› 4inf

X-ray diffraction
1.48Å resolution

Crystal structure of amidohydrolase saro_0799 (target efi-505250) from novosphingobium aromaticivorans dsm 12444 with bound calcium

Released:
DOI: 10.2210/pdb4inf/pdb
PDB entry 4inf coloured by chain, front view.
PDB entry 4inf coloured by chain, side view.
PDB entry 4inf coloured by chain, top view.
Source organism: Novosphingobium aromaticivorans DSM 12444
Entry authors: Patskovsky Y, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Raushel FM, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 373 amino acids
Theoretical weight: 42.46 KDa
Source organism: Novosphingobium aromaticivorans DSM 12444
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2GA79 (Residues: 1-351; Coverage: 100%)
Gene name: Saro_0799
Sequence domains: Amidohydrolase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

4 bound ligands:
Ligand CA 4 x CA
Ligand CL 16 x CL
Ligand OXD 4 x OXD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 92.945Å b: 61.024Å c: 131.385Å
α: 90° β: 103.35° γ: 90°
R-values:
R R work R free
0.143 0.141 0.19
Expression system: Escherichia coli BL21(DE3)

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