› 4ipl

X-ray diffraction
2Å resolution

The crystal structure of 6-phospho-beta-glucosidase BglA-2 from Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb4ipl/pdb
PDB entry 4ipl coloured by chain, front view.
PDB entry 4ipl coloured by chain, side view.
PDB entry 4ipl coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Structural insights into the substrate specificity of a 6-phospho-β-glucosidase BglA-2 from Streptococcus pneumoniae TIGR4.
Yu WL, Jiang YL, Pikis A, Cheng W, Bai XH, Ren YM, Thompson J, Zhou CZ, Chen Y
J Biol Chem 288 14949-58 (2013)
PMID: 23580646

Function and Biology Details

Reaction catalysed: 
6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H(2)O = D-glucose + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phospho-beta-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 487 amino acids
Theoretical weight: 56.23 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0H2UP35 (Residues: 1-471; Coverage: 100%)
Gene names: SP_0578, bglA-2
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 183.28Å b: 65.35Å c: 126.69Å
α: 90° β: 133.38° γ: 90°
R-values:
R R work R free
0.179 0.177 0.222
Expression system: Escherichia coli BL21

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Citations

1 review citation

Glycan-metabolizing enzymes in microbe-host interactions: the Streptococcus pneumoniae paradigm.
Hobbs et al. (2018)
13 other articles

1 mention without citation

Structural insights into the substrate specificity of a 6-phospho-β-glucosidase BglA-2 from Streptococcus pneumoniae TIGR4.
Yu et al. (2013)