› 4iqe

X-ray diffraction
2.5Å resolution

Crystal Structure of Carboxyvinyl-Carboxyphosphonate Phosphorylmutase from Bacillus anthracis str. Ames Ancestor

Released:
DOI: 10.2210/pdb4iqe/pdb
PDB entry 4iqe coloured by chain, front view.
PDB entry 4iqe coloured by chain, side view.
PDB entry 4iqe coloured by chain, top view.
Source organism: Bacillus anthracis
Entry authors: Kim Y, Maltseva N, Kwon K, Anderson WF, Joachimiak A, CSGID, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-105699 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylisocitrate lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 33.65 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A6L7HJ56 (Residues: 1-302; Coverage: 100%)
Gene names: GBAA_2350, prpB
Sequence domains: Phosphoenolpyruvate phosphomutase
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3221
Unit cell:
a: 106.166Å b: 106.166Å c: 113.449Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.159 0.178
Expression system: Escherichia coli BL21

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