› 4iqf

X-ray diffraction
2.38Å resolution

Crystal Structure of Methyionyl-tRNA Formyltransferase from Bacillus anthracis

Released:
DOI: 10.2210/pdb4iqf/pdb
PDB entry 4iqf coloured by chain, front view.
PDB entry 4iqf coloured by chain, side view.
PDB entry 4iqf coloured by chain, top view.
Source organism: Bacillus anthracis
Entry authors: Kim Y, Makowska-Grzyska M, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionyl-tRNA formyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 317 amino acids
Theoretical weight: 35.33 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81WH2 (Residues: 1-314; Coverage: 100%)
Gene names: BAS3717, BA_4004, GBAA_4004, fmt
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PG5 1 x PG5
Ligand GOL 4 x GOL
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 62.856Å b: 191.501Å c: 89.946Å
α: 90° β: 89.95° γ: 90°
R-values:
R R work R free
0.183 0.181 0.21
Expression system: Escherichia coli

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