› 4iqi

X-ray diffraction
1.5Å resolution

Crystal Structure of 7-cyano-7-deazaguanine Reductase, QueF from Vibrio cholerae O1 biovar El Tor complexed with cytosine

Released:
DOI: 10.2210/pdb4iqi/pdb
PDB entry 4iqi coloured by chain, front view.
PDB entry 4iqi coloured by chain, side view.
PDB entry 4iqi coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Kim Y, Zhou M, Gu M, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-carbaguanine + 2 NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH-dependent 7-cyano-7-deazaguanine reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 33.32 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KTK0 (Residues: 1-281; Coverage: 100%)
Gene names: VC_0902, queF
Sequence domains:
Structure domains: GTP Cyclohydrolase I, domain 2

Ligands and Environments

3 bound ligands:
Ligand CYT 2 x CYT
Ligand CL 2 x CL
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 96.122Å b: 90.936Å c: 73.166Å
α: 90° β: 90.1° γ: 90°
R-values:
R R work R free
0.154 0.151 0.172
Expression system: Escherichia coli

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