› 4iqx

X-ray diffraction
2.5Å resolution

Mutant P44S P169S M296I of Foot-and-mouth disease Virus RNA-dependent RNA polymerase

Released:
DOI: 10.2210/pdb4iqx/pdb
PDB entry 4iqx coloured by chain, front view.
PDB entry 4iqx coloured by chain, side view.
PDB entry 4iqx coloured by chain, top view.
Source organisms:
Primary publication:
A multi-step process of viral adaptation to a mutagenic nucleoside analogue by modulation of transition types leads to extinction-escape.
Agudo R, Ferrer-Orta C, Arias A, de la Higuera I, Perales C, Pérez-Luque R, Verdaguer N, Domingo E
PLoS Pathog 6 e1001072 (2010)
PMID: 20865120

Function and Biology Details

Reactions catalysed: 
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193000 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
3D polymerase Chain: A
Molecule details ›
Chain: A
Length: 481 amino acids
Theoretical weight: 54.08 KDa
Source organism: Foot and mouth disease virus C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9QCE4 (Residues: 1858-2327; Coverage: 20%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:
RNA (5'-R(*AP*UP*GP*GP*GP*CP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.24 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*GP*GP*CP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 5 nucleotides
Theoretical weight: 1.56 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 93.836Å b: 93.836Å c: 99.867Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.222 0.254
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

Viral quasispecies evolution.
Domingo et al. (2012)
18 more

1 mention without citation

Contribution of a Multifunctional Polymerase Region of Foot-and-Mouth Disease Virus to Lethal Mutagenesis.
de la Higuera et al. (2018)