› 4ire

X-ray diffraction
3.19Å resolution

Crystal structure of GLIC with mutations at the loop C region

Released:
DOI: 10.2210/pdb4ire/pdb
PDB entry 4ire coloured by chain, front view.
PDB entry 4ire coloured by chain, side view.
PDB entry 4ire coloured by chain, top view.
Source organism: Gloeobacter violaceus PCC 7421
Primary publication:
Signal transduction pathways in the pentameric ligand-gated ion channels.
Mowrey D, Chen Q, Liang Y, Liang J, Xu Y, Tang P
PLoS One 8 e64326 (2013)
PMID: 23667707

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-gated ion channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 317 amino acids
Theoretical weight: 36.27 KDa
Source organism: Gloeobacter violaceus PCC 7421
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NDN8 (Residues: 43-359; Coverage: 100%)
Gene names: glr4197, glvI
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand LMD 6 x LMD
Ligand PC1 10 x PC1
Ligand OXL 17 x OXL
Ligand ACT 10 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 182.03Å b: 133.62Å c: 161.43Å
α: 90° β: 102.57° γ: 90°
R-values:
R R work R free
0.204 0.204 0.243
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Ethanol effects on glycinergic transmission: From molecular pharmacology to behavior responses.
Burgos et al. (2015)
1 more

4 mentions without citation

Prediction and validation of protein intermediate states from structurally rich ensembles and coarse-grained simulations.
Orellana et al. (2016)
3 more