› 4isa

X-ray diffraction
1.8Å resolution

Crystal Structure of the Escherichia coli LpxC/BB-78485 complex

Released:
DOI: 10.2210/pdb4isa/pdb
PDB entry 4isa coloured by chain, front view.
PDB entry 4isa coloured by chain, side view.
PDB entry 4isa coloured by chain, top view.
Source organism: Escherichia coli IHE3034
Primary publication:
Structural basis of the promiscuous inhibitor susceptibility of Escherichia coli LpxC.
Lee CJ, Liang X, Gopalaswamy R, Najeeb J, Ark ED, Toone EJ, Zhou P
ACS Chem Biol 9 237-46 (2014)
PMID: 24117400

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 33.55 KDa
Source organism: Escherichia coli IHE3034
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand GVR 2 x GVR
Ligand FMT 17 x FMT
Ligand D1D 1 x D1D
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P61
Unit cell:
a: 106.788Å b: 106.788Å c: 52.474Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The Power of Asymmetry: Architecture and Assembly of the Gram-Negative Outer Membrane Lipid Bilayer.
Henderson et al. (2016)
4 more

2 mentions without citation

Structural basis of the promiscuous inhibitor susceptibility of Escherichia coli LpxC.
Lee et al. (2014)
1 more