› 4isd

X-ray diffraction
2.65Å resolution

Crystal structure of GLUTATHIONE TRANSFERASE homolog from BURKHOLDERIA GL BGR1, TARGET EFI-501803, with bound glutathione

Released:
DOI: 10.2210/pdb4isd/pdb
PDB entry 4isd coloured by chain, front view.
PDB entry 4isd coloured by chain, side view.
PDB entry 4isd coloured by chain, top view.
Source organism: Burkholderia glumae BGR1
Entry authors: Vetting MW, Toro R, Bhosle R, Al Obaidi NF, Morisco LL, Wasserman SR, Sojitra S, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Hillerich B, Love J, Seidel RD, Imker HJ, Armstrong RN, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GST N-terminal domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 220 amino acids
Theoretical weight: 25.24 KDa
Source organism: Burkholderia glumae BGR1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5A7Z5 (Residues: 1-211; Coverage: 100%)
Gene name: bglu_1g21330
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GSH 5 x GSH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2221
Unit cell:
a: 56.004Å b: 196.343Å c: 275.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links