› 4it1

X-ray diffraction
2.2Å resolution

Crystal structure of enolase pfl01_3283 (target efi-502286) from pseudomonas fluorescens pf0-1 with bound magnesium, potassium and tartrate

Released:
DOI: 10.2210/pdb4it1/pdb
PDB entry 4it1 coloured by chain, front view.
PDB entry 4it1 coloured by chain, side view.
PDB entry 4it1 coloured by chain, top view.
Source organism: Pseudomonas fluorescens Pf0-1
Entry authors: Patskovsky Y, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Al Obaidi N, Stead M, Love J, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-174706 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glucarate dehydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 446 amino acids
Theoretical weight: 49.49 KDa
Source organism: Pseudomonas fluorescens Pf0-1
Expression system: Not provided
UniProt:
  • Canonical: Q3KB33 (Residues: 1-424; Coverage: 100%)
Gene name: Pfl01_3283
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand TLA 4 x TLA
Ligand K 2 x K
Ligand BCT 1 x BCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 82.321Å b: 147.695Å c: 173.195Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.25
Expression system: Not provided

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