› 4it2

X-ray diffraction
2.1Å resolution

Mn(III)-PPIX bound Tt H-NOX

Released:
DOI: 10.2210/pdb4it2/pdb
PDB entry 4it2 coloured by chain, front view.
PDB entry 4it2 coloured by chain, side view.
PDB entry 4it2 coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Porphyrin-substituted H-NOX proteins as high-relaxivity MRI contrast agents.
Winter MB, Klemm PJ, Phillips-Piro CM, Raymond KN, Marletta MA
Inorg Chem 52 2277-9 (2013)
PMID: 23394479

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting transducer domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 22.05 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RBX6 (Residues: 1-188; Coverage: 31%)
Gene names: TTE0680, Tar4
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

1 bound ligand:
Ligand MNH 3 x MNH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 120.245Å b: 45.416Å c: 95.743Å
α: 90° β: 122.82° γ: 90°
R-values:
R R work R free
0.211 0.209 0.245
Expression system: Escherichia coli

Quick links

Citations

3 review citations

A review of responsive MRI contrast agents: 2005-2014.
Hingorani et al. (2015)
2 more

1 mention without citation

Porphyrin-substituted H-NOX proteins as high-relaxivity MRI contrast agents.
Winter et al. (2013)