› 4itq

X-ray diffraction
2.7Å resolution

Crystal structure of hypothetical protein SCO1480 bound to DNA

Released:
DOI: 10.2210/pdb4itq/pdb
PDB entry 4itq coloured by chain, front view.
PDB entry 4itq coloured by chain, side view.
PDB entry 4itq coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
A novel nucleoid-associated protein specific to the actinobacteria.
Swiercz JP, Nanji T, Gloyd M, Guarné A, Elliot MA
Nucleic Acids Res 41 4171-84 (2013)
PMID: 23427309

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117662 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Integration host factor Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 11.72 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KXR9 (Residues: 1-107; Coverage: 100%)
Gene names: SC9C5.04c, SCO1480, sihF
Structure domains: Helicase, Ruva Protein; domain 3
5'-D(P*CP*CP*GP*CP*GP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.39 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Not provided
5'-D(P*GP*CP*GP*CP*GP*CP*GP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 8 nucleotides
Theoretical weight: 2.47 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 43.42Å b: 71.782Å c: 102.925Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.262 0.315
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Taxonomy, Physiology, and Natural Products of Actinobacteria.
Barka et al. (2016)
6 more

4 mentions without citation

A novel nucleoid-associated protein specific to the actinobacteria.
Swiercz et al. (2013)
3 more