› 4iu2

X-ray diffraction
2Å resolution

Cohesin-dockerin -X domain complex from Ruminococcus flavefacience

Released:
DOI: 10.2210/pdb4iu2/pdb
PDB entry 4iu2 coloured by chain, front view.
PDB entry 4iu2 coloured by chain, side view.
PDB entry 4iu2 coloured by chain, top view.
Source organism: Ruminococcus flavefaciens
Primary publication:
Atypical cohesin-dockerin complex responsible for cell surface attachment of cellulosomal components: binding fidelity, promiscuity, and structural buttresses.
Salama-Alber O, Jobby MK, Chitayat S, Smith SP, White BA, Shimon LJW, Lamed R, Frolow F, Bayer EA
J Biol Chem 288 16827-16838 (2013)
PMID: 23580648

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106044 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell-wall anchoring protein Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 22.95 KDa
Source organism: Ruminococcus flavefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0AEF6 (Residues: 29-230; Coverage: 67%)
Gene name: scaE
Structure domains: Immunoglobulin-like
Cellulose-binding protein CttA N-terminal domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 243 amino acids
Theoretical weight: 26.6 KDa
Source organism: Ruminococcus flavefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0AEF5 (Residues: 565-803; Coverage: 31%)
Gene name: cttA
Sequence domains: Cellulose-binding protein CttA N-terminal domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand SO4 5 x SO4
Ligand CL 3 x CL
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P43212
Unit cell:
a: 78.714Å b: 78.714Å c: 203.238Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.196
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Cellulosomes: bacterial nanomachines for dismantling plant polysaccharides.
Artzi et al. (2017)
4 more

5 mentions without citation

Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass.
Kameshwar et al. (2019)
4 more