› 4iu8

X-ray diffraction
3.11Å resolution

Crystal structure of a membrane transporter (selenomethionine derivative)

Released:
DOI: 10.2210/pdb4iu8/pdb
PDB entry 4iu8 coloured by chain, front view.
PDB entry 4iu8 coloured by chain, side view.
PDB entry 4iu8 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure and mechanism of a nitrate transporter.
Yan H, Huang W, Yan C, Gong X, Jiang S, Zhao Y, Wang J, Shi Y
Cell Rep 3 716-23 (2013)
PMID: 23523348

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrate/nitrite transporter NarU Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 51.36 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37758 (Residues: 1-462; Coverage: 100%)
Gene names: JW1464, b1469, narU, yddF
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

1 bound ligand:
Ligand NO3 1 x NO3
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 106.582Å b: 117.501Å c: 127.411Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.239 0.299
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structural Biology of the Major Facilitator Superfamily Transporters.
Yan N. (2015)
11 more

4 mentions without citation

Structural basis for dynamic mechanism of nitrate/nitrite antiport by NarK.
Fukuda et al. (2015)
3 more