› 4iu9

X-ray diffraction
3Å resolution

Crystal structure of a membrane transporter

Released:
DOI: 10.2210/pdb4iu9/pdb
PDB entry 4iu9 coloured by chain, front view.
PDB entry 4iu9 coloured by chain, side view.
PDB entry 4iu9 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure and mechanism of a nitrate transporter.
Yan H, Huang W, Yan C, Gong X, Jiang S, Zhao Y, Wang J, Shi Y
Cell Rep 3 716-23 (2013)
PMID: 23523348

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrate/nitrite transporter NarU Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 50.65 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37758 (Residues: 1-462; Coverage: 100%)
Gene names: JW1464, b1469, narU, yddF
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 117.439Å b: 118.211Å c: 127.056Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.261 0.316
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
11 more

3 mentions without citation

Delineating the extracellular water-accessible surface of the proton-coupled folate transporter.
Duddempudi et al. (2013)
2 more