› 4iuf

X-ray diffraction
2.75Å resolution

Crystal Structure of Human TDP-43 RRM1 Domain in Complex with a Single-stranded DNA

Released:
DOI: 10.2210/pdb4iuf/pdb
PDB entry 4iuf coloured by chain, front view.
PDB entry 4iuf coloured by chain, side view.
PDB entry 4iuf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of TDP-43 RRM1-DNA complex reveals the specific recognition for UG- and TG-rich nucleic acids.
Kuo PH, Chiang CH, Wang YT, Doudeva LG, Yuan HS
Nucleic Acids Res 42 4712-22 (2014)
PMID: 24464995

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117663 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
TAR DNA-binding protein 43 Chain: A
Molecule details ›
Chain: A
Length: 77 amino acids
Theoretical weight: 8.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13148 (Residues: 103-179; Coverage: 19%)
Gene names: TARDBP, TDP43
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
5'-D(*GP*TP*TP*GP*(XUA)P*GP*CP*GP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.88 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand XUA 1 x XUA

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P6522
Unit cell:
a: 71.11Å b: 71.11Å c: 101.663Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.206 0.259
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

27 review citations

Molecular Mechanisms of TDP-43 Misfolding and Pathology in Amyotrophic Lateral Sclerosis.
Prasad et al. (2019)
26 more

14 mentions without citation

The structure, function and evolution of proteins that bind DNA and RNA.
Hudson et al. (2014)
13 more