› 4iw7

X-ray diffraction
2.25Å resolution

Crystal structure of 8-amino-7-oxononanoate synthase (bioF) from Francisella tularensis.

Released:
DOI: 10.2210/pdb4iw7/pdb
PDB entry 4iw7 coloured by chain, front view.
PDB entry 4iw7 coloured by chain, side view.
PDB entry 4iw7 coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Entry authors: Newcomb W, Niedzialkowska E, Porebski PJ, Grimshaw S, Anderson WF, Minor W, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO(2) + holo-[acyl-carrier protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class I/classII domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 45.42 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NGB3 (Residues: 1-375; Coverage: 100%)
Gene names: FTT_0936c, bioF
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: R32
Unit cell:
a: 113.486Å b: 113.486Å c: 158.128Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.238
Expression system: Escherichia coli BL21(DE3)

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