› 4iwb

X-ray diffraction
1.75Å resolution

Novel Fold of FliC/FliS Fusion Protein

Released:
DOI: 10.2210/pdb4iwb/pdb
PDB entry 4iwb coloured by chain, front view.
PDB entry 4iwb coloured by chain, side view.
PDB entry 4iwb coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Protein design by fusion: implications for protein structure prediction and evolution.
Skorupka K, Han SK, Nam HJ, Kim S, Faham S
Acta Crystallogr D Biol Crystallogr 69 2451-60 (2013)
PMID: 24311586

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flagellar protein FliS; Flagellin Chains: A, B
Molecule details ›
Chains: A, B
Length: 165 amino acids
Theoretical weight: 19.29 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67803 (Residues: 478-518; Coverage: 8%)
  • Canonical: O67806 (Residues: 5-124; Coverage: 97%)
Gene names: aq_1998, aq_2002, flaA, fliC, fliS
Sequence domains:
Structure domains: Flagellar protein FliS

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 61.129Å b: 66.157Å c: 97.169Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.232
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Protein rethreading: A novel approach to protein design.
Agah et al. (2016)

2 mentions without citation

Structural Conservation and Adaptation of the Bacterial Flagella Motor.
Carroll et al. (2020)
1 more