› 4iwh

X-ray diffraction
1.75Å resolution

Crystal structure of a 3-isopropylmalate dehydrogenase from Burkholderia pseudomallei

Released:
DOI: 10.2210/pdb4iwh/pdb
PDB entry 4iwh coloured by chain, front view.
PDB entry 4iwh coloured by chain, side view.
PDB entry 4iwh coloured by chain, top view.
Source organism: Burkholderia thailandensis E264
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
(1a) (2R,3S)-3-isopropylmalate + NAD(+) = (2S)-2-isopropyl-3-oxosuccinate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174022 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-isopropylmalate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 363 amino acids
Theoretical weight: 39.32 KDa
Source organism: Burkholderia thailandensis E264
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2T7H6 (Residues: 1-355; Coverage: 100%)
Gene names: BTH_II0674, leuB
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 141.6Å b: 60.1Å c: 103.19Å
α: 90° β: 112.86° γ: 90°
R-values:
R R work R free
0.169 0.168 0.201
Expression system: Escherichia coli

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