› 4ix7

X-ray diffraction
1.58Å resolution

Crystal Structure of the insv-BEN domain complexed to its DNA target site

Released:
DOI: 10.2210/pdb4ix7/pdb
PDB entry 4ix7 coloured by chain, front view.
PDB entry 4ix7 coloured by chain, side view.
PDB entry 4ix7 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors.
Dai Q, Ren A, Westholm JO, Serganov AA, Patel DJ, Lai EC
Genes Dev 27 602-14 (2013)
PMID: 23468431

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117666 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protein insensitive Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 13.05 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8SYK5 (Residues: 251-365; Coverage: 31%)
Gene names: CG3227, insv
Sequence domains: BEN domain
Structure domains: BEN domain
5'-D(*GP*TP*TP*CP*CP*AP*AP*TP*TP*GP*GP*AP*A)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 13 nucleotides
Theoretical weight: 3.99 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 43.382Å b: 75.464Å c: 89.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.216
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Making connections: insulators organize eukaryotic chromosomes into independent cis-regulatory networks.
Chetverina et al. (2014)
5 more

8 mentions without citation

The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors.
Dai et al. (2013)
7 more