› 4iyg

X-ray diffraction
2.7Å resolution

Structure of strictosidine synthase in complex with 2-(1H-INDOL-3-YL)-N-METHYLETHANAMINE

Released:
DOI: 10.2210/pdb4iyg/pdb
PDB entry 4iyg coloured by chain, front view.
PDB entry 4iyg coloured by chain, side view.
PDB entry 4iyg coloured by chain, top view.
Source organism: Rauvolfia serpentina
Primary publication:
Using Strictosidine Synthase to Prepare Novel Alkaloids.
Zhu H, Kerčmar P, Wu F, Rajendran C, Sun L, Wang M, Stöckigt J
Curr Med Chem (2015)
PMID: 25850764

Function and Biology Details

Reaction catalysed: 
3-alpha-(S)-strictosidine + H(2)O = tryptamine + secologanin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159353 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strictosidine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 33.69 KDa
Source organism: Rauvolfia serpentina
Expression system: Escherichia coli
UniProt:
  • Canonical: P68175 (Residues: 32-333; Coverage: 94%)
Gene name: STR1
Sequence domains: Strictosidine synthase
Structure domains: TolB, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand 1HU 1 x 1HU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: R3
Unit cell:
a: 150.23Å b: 150.23Å c: 122.101Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.252
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Pictet-Spengler Reaction Updates Its Habits.
Calcaterra et al. (2020)
1 more

1 mention without citation

Profiles of Metabolic Genes in Uncaria rhynchophylla and Characterization of the Critical Enzyme Involved in the Biosynthesis of Bioactive Compounds-(iso)Rhynchophylline.
Yang et al. (2022)