› 4iyh

X-ray diffraction
1.88Å resolution

The crystal structure of a secreted protein EsxB (SeMet-labeled, C-term. His-Tagged) from Bacillus anthracis str. Sterne

Released:
DOI: 10.2210/pdb4iyh/pdb
PDB entry 4iyh coloured by chain, front view.
PDB entry 4iyh coloured by chain, side view.
PDB entry 4iyh coloured by chain, top view.
Source organism: Bacillus anthracis str. Sterne
Entry authors: Fan Y, Tan K, Chhor G, Jedrzejczak R, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-105702 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ESAT-6-like protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 98 amino acids
Theoretical weight: 11.15 KDa
Source organism: Bacillus anthracis str. Sterne
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6L7HJC4 (Residues: 1-90; Coverage: 100%)
Gene name: GBAA_2191
Sequence domains: Proteins of 100 residues with WXG
Structure domains: HP0062-like domain

Ligands and Environments

4 bound ligands:
Ligand EDO 2 x EDO
Ligand FMT 3 x FMT
Ligand ACT 2 x ACT
Ligand SO4 7 x SO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P31
Unit cell:
a: 77.337Å b: 77.337Å c: 71.226Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.145 0.141 0.162
Expression system: Escherichia coli BL21(DE3)

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