› 4j19

X-ray diffraction
2.9Å resolution

Structure of a novel telomere repeat binding protein bound to DNA

Released:
DOI: 10.2210/pdb4j19/pdb
PDB entry 4j19 coloured by chain, front view.
PDB entry 4j19 coloured by chain, side view.
PDB entry 4j19 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment.
Kappei D, Butter F, Benda C, Scheibe M, Draškovič I, Stevense M, Novo CL, Basquin C, Araki M, Araki K, Krastev DB, Kittler R, Jessberger R, Londoño-Vallejo JA, Mann M, Buchholz F
EMBO J 32 1681-701 (2013)
PMID: 23685356

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117671 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeobox-containing protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 13.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6NT76 (Residues: 233-345; Coverage: 27%)
Gene names: HMBOX1, HOT1, TAH1
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*CP*TP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.95 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*CP*TP*AP*AP*CP*CP*CP*TP*AP*AP*CP*CP*CP*TP*AP*AP*CP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.68 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 111.433Å b: 116.49Å c: 75.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.221
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Cell biology of disease: Telomeropathies: an emerging spectrum disorder.
Holohan et al. (2014)
11 more

6 mentions without citation

Dual kinase-bromodomain inhibitors for rationally designed polypharmacology.
Ciceri et al. (2014)
5 more