› 4j40

X-ray diffraction
2.99Å resolution

Crystal structure of the dual-domain GGDEF-EAL module of FimX from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4j40/pdb
PDB entry 4j40 coloured by chain, front view.
PDB entry 4j40 coloured by chain, side view.
PDB entry 4j40 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural analysis of the GGDEF-EAL domain-containing c-di-GMP receptor FimX.
Navarro MV, De N, Bae N, Wang Q, Sondermann H
Structure 17 1104-16 (2009)
PMID: 19679088

Function and Biology Details

Reaction catalysed: 
Cyclic di-3',5'-guanylate + H(2)O = 5'-phosphoguanylyl(3'->5')guanosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190964 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FimX Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 47.75 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HUK6 (Residues: 260-691; Coverage: 63%)
Gene names: PA4959, fimX
Sequence domains:
Structure domains: EAL domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P41212
Unit cell:
a: 106.251Å b: 106.251Å c: 193.351Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
24 more