› 4j5m

X-ray diffraction
2.07Å resolution

Structure of the Cargo Binding Domain from Human Myosin Vb

Released:
DOI: 10.2210/pdb4j5m/pdb
PDB entry 4j5m coloured by chain, front view.
PDB entry 4j5m coloured by chain, side view.
PDB entry 4j5m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into functional overlapping and differentiation among myosin V motors.
Nascimento AFZ, Trindade DM, Tonoli CCC, de Giuseppe PO, Assis LHP, Honorato RV, de Oliveira PSL, Mahajan P, Burgess-Brown NA, von Delft F, Larson RE, Murakami MT
J Biol Chem 288 34131-34145 (2013)
PMID: 24097982

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Unconventional myosin-Vb Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 45.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ULV0 (Residues: 1453-1848; Coverage: 21%)
Gene names: KIAA1119, MYO5B
Sequence domains: DIL domain

Ligands and Environments

1 bound ligand:
Ligand NO3 2 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2221
Unit cell:
a: 69.093Å b: 78.2Å c: 151.817Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Myosin-Driven Intracellular Transport.
Titus MA. (2018)
6 more

7 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
6 more