› 4j5t

X-ray diffraction
2.04Å resolution

Crystal structure of Processing alpha-Glucosidase I

Released:
DOI: 10.2210/pdb4j5t/pdb
PDB entry 4j5t coloured by chain, front view.
PDB entry 4j5t coloured by chain, side view.
PDB entry 4j5t coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Specificity of Processing α-glucosidase I is guided by the substrate conformation: crystallographic and in silico studies.
Barker MK, Rose DR
J Biol Chem 288 13563-74 (2013)
PMID: 23536181

Function and Biology Details

Reaction catalysed: 
Glc(3)Man(9)GlcNAc(2)-[protein] + H(2)O = Glc(2)Man(9)GlcNAc(2)-[protein] + beta-D-glucopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Mannosyl-oligosaccharide glucosidase Chain: A
Molecule details ›
Chain: A
Length: 811 amino acids
Theoretical weight: 94.28 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P53008 (Residues: 35-833; Coverage: 96%)
Gene names: CWH41, GLS1, YGL027C
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 94.8Å b: 101.8Å c: 103.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.235
Expression system: Komagataella pastoris

Quick links

Citations

8 review citations

N-linked sugar-regulated protein folding and quality control in the ER.
Tannous et al. (2015)
7 more

16 mentions without citation

Advanced Bioinformatics Tools in the Pharmacokinetic Profiles of Natural and Synthetic Compounds with Anti-Diabetic Activity.
Udrea et al. (2021)
15 more