› 4j7n

X-ray diffraction
1.5Å resolution

Crystal structure of mouse DXO in complex with M7GPPPG cap

Released:
DOI: 10.2210/pdb4j7n/pdb
PDB entry 4j7n coloured by chain, front view.
PDB entry 4j7n coloured by chain, side view.
PDB entry 4j7n coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A mammalian pre-mRNA 5' end capping quality control mechanism and an unexpected link of capping to pre-mRNA processing.
Jiao X, Chang JH, Kilic T, Tong L, Kiledjian M
Mol Cell 50 104-15 (2013)
PMID: 23523372

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Decapping and exoribonuclease protein Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 43.13 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O70348 (Residues: 27-384; Coverage: 90%)
Gene names: Dom3z, Dxo, Ng6
Sequence domains: RAI1 like PD-(D/E)XK nuclease

Ligands and Environments

3 bound ligands:
Ligand GTG 2 x GTG
Ligand 9MG 1 x 9MG
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 49.91Å b: 87.46Å c: 53.27Å
α: 90° β: 113.69° γ: 90°
R-values:
R R work R free
0.18 0.172 0.2
Expression system: Escherichia coli

Quick links

Citations

36 review citations

Coupling mRNA processing with transcription in time and space.
Bentley DL. (2014)
35 more

3 mentions without citation

A mammalian pre-mRNA 5' end capping quality control mechanism and an unexpected link of capping to pre-mRNA processing.
Jiao et al. (2013)
2 more